My tutorial, SAMtools: A Primer is now available online. My hope is that the primer eases some of the “information pain” usually associated with learning next-generation sequence analysis. To that end, the primer is completely self-contained — you only need to install SAMtools, and the tutorial section walks you through a complete next-generation sequence analysis work-flow.
Samtools, the Swiss Army knife of next-gen sequencing. Image source: Wikipedia
Sample data sets are provided on the companion github repository, and a short glossary is also included at the end of the document.
If you identify any errors or have suggestions for improving the primer, I would love to hear from you. Just drop me an email at: cerami AT gmail.com.
August, 2013 Update: The Primer now has a new section where you can immediately post feedback and questions. Thanks for all the feedback so far!